involvement of microrna-133 and -29 in cardiac disturbances in diabetic ovariectomized rats

Authors

parisa habibi department of physiology, tabriz university of medical sciences, tabriz, iran

alireza alihemmati department of histology & embryology, tabriz university of medical sciences, tabriz, iran

mohammadreza nasirzadeh department of physiology, tabriz branch, islamic azad university, tabriz, iran

hadi yousefi department of physiology, tabriz university of medical sciences, tabriz, iran

abstract

objective(s): menopause and diabetes obviously increase the risk of cardiovascular disease in women. the aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of mirna-29, mirna-133, igf-1 and bcl-2 genes and bcl-2 protein and caspase 3 activity in the hearts of female rats. materials and methods: forty female wistar rats were divided into four groups: control, sham, ovariectomized (ovx), and ovariectomized with type 2 diabetes (ovx.d). after the 8-week experiment, the histological evaluation of the heart tissue was performed using h&e; staining and pas analysis, and cardiac expression of mirna-29, mirna-133, igf-1, and bcl-2 were evaluated using real-time pcr, and bcl-2 protein and caspase 3 activity were evaluated using western blot and elisa. results: ovariectomy significantly decreased mirna-29, mirna-133, igf-1, and bcl-2 expression and bcl-2 protein and increased caspase 3 activity in the heart compared to sham animals group (p<0.05). type 2 diabetes in ovariectomized rats markedly decreased expression of mirna-29, mirna-133, igf-1, bcl-2 genes, and bcl-2 protein, and increased caspase 3 activity and reduced collagen and fibroblast tissue and glycogen granule deposition in relation to ovx group (p<0.05). conclusion: our findings suggest that type 2 diabetes and menopause synergically could enhance the cardiac fibrosis through dysregulation of mirna-29, mirna-133, igf-1, and bcl-2 genes expression and bcl-2 protein and upregulation of caspase 3 activity.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats

Objective(s): Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspase 3 activity in the hearts of female rats. Materials and Methods: Forty Female Wistar rats were ...

full text

Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats

OBJECTIVES Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspase 3 activity in the hearts of female rats. MATERIALS AND METHODS Forty Female Wistar rats were div...

full text

Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats

Objective(s): The beneficial and more potent role of exercise to prevent heart apoptosis in ovariectomized rats has been known. The aim of this study was to examine the effects of swimming training on cardiac expression of Bcl-2, and Mir-133 levels and glycogen changes in the myocyte. Materials and Methods: Forty animals were separated into four groups as control, sham, ovariectomy (OVX) and o...

full text

The role of microRNA-133 in cardiac hypertrophy uncovered.

Studies of microRNAs (miRNAs) have recently uncovered a new level of intricacy in the regulation of gene expression during organogenesis and pathogenesis.1 miR133, which is enriched in cardiac and skeletal muscle, is involved in cell specification,2 differentiation,3 and development.4 It is also downregulated during cardiac hypertrophy,5–7 which suggested that it may play a role in the underlyi...

full text

role of cultural iran in promotion of multilaieralism in central asia and caucasus

cultural iran is a scope that is more extended than the political territories of iran as a political unit. this concept means that cultural geography(mehdi moghanlo-1383-1) of iran is greater than its political geography which, according to history, has a long history extending west-east from kandahar to the euphrates and north-south from the persian gulf to the caucasus including transoxiana a...

15 صفحه اول

study the effects of low level laser diode on no production and healing of diabetic and cortisone acetate and normal wounded sd rats

nitric oxide (no) is a small molecule synthesized by most of mammalian cells with diverse biological activities including vasodilatation, host defense and wound healing. impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. on the other hand the application of laser in biomedical area have been increased. thus, the current studies were desi...

15 صفحه اول

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۹، شماره ۱۱، صفحات ۱۱۷۷-۱۱۸۵

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023